杨福全研究员
Prof. Fuquan Yang

简历：
1983.09-1987.07  郑州大学化学系，理学学士
1987.09-1992.12  中国科学院兰州化学物理研究所，理学博士
1992.12-1995.06  日本环境厅国立环境研究所环境化学部，博士后
1995.06-2000.12  北京新技术应用研究所，北京天然产物分离纯化技术研究中心副研究员, 副主任
2000.12-2004.06  访问学者,美国国家卫生研究院（NIH）国立心、肺和血液研究所(NHLBI)访问学者
2006.07-2006.08  美国Scripps研究所，高级访问学者
2004-至今：      中国科学院生物物理所，研究员，质谱首席技术专家，博士生导师

研究方向：
    1、疾病蛋白质组学研究与疾病相关蛋白质生物标志物的发现与鉴定
    2、膜蛋白质组学和亚细胞器蛋白质组学——技术与应用
    3、蛋白质翻译后修饰——技术与应用
    4、蛋白质复合物的分析鉴定与蛋白质-蛋白质相互作用：技术与应用
    5、定量蛋白质组学：技术与应用

Biography & Introduction

Education and Research Experience:

1987: Zhengzhou University, Zhengzhou, China, Bachelor of Chemistry.
1992: Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China. Ph. D in Analytical Chemistry.
1992-1995: National Institute for Environmental Studies, Japan. Post-doctoral fellow.1995-2000: Beijing Institute of New Technology Application, Beijing, China, Associate professor.
2000-2004: National Heart, Lung and Blood Institute, National Institutes of Health, MD, USA. Visiting fellow.
2006.7-2006.8: The Scripps Research Institute, CA, USA. Visiting scientist.

Research interests
1. Discovery and identification of potential protein biomarkers
2. Identification of protein complex: technique and application.
3. Proteomics of membrane and subcellular organelles
4. Quantitative proteomics: technique and application
5. Identification of post-translational modification: technique and application
6. MS-based peptidomics: technique and application
7. MS-based lipidomics: technique and application
8. Separation science: technique and application

Current work
1. Development of new methods and technique for high-throughput, sensitive identification of protein complex, membrane protein, potential protein biomarkers and low abundant proteins from biological sample. We also focus on the discovery and identification of potential protein biomarkers.
2. Development of new methods and technique for high-throughput, sensitive identification of post-translational modification, including protein phosphorylation, glycosylation. We also focus on the study on histone modifications.
3. Development of new methods and technique for quantatitive proteomics and targeted proteomics. We also focus on the application of quantatitive proteomics in biology.

Seleted Publications

1. J. J. Hou, Z. Xie, P. Xue, Z.  Cui, X.  Chen, J. Li, T. Cai, P. Wu and F. Q. Yang*, Phosphoproteome Analysis of Rat L6 Myotubes Using Reversed-Phase C18 Prefractionation and Titanium Dioxide Enrichment, J Proteome Res, 2010, 9, 777–788.

2. Jing Li, Tanxi Cai, Peng Wu, Ziyou Cui, Xiulan Chen, Junjie Hou, Zhensheng Xie, Peng Xue, Linan Shi, Pingsheng Liu, John R. Yates III, Fuquan Yang*, Proteomic analysis of the mitochondria from C. elegans, Proteomics, 2009, 9, 4539-4553.
 
3. Z. Y. Cui, X. L. Chen, B. W. Lu, S. K. Park, T. Xu, Z. S. Xie, P. Xue, J. J. Hou, H. Y. Hang, J. R. Yates III* and F. Q. Yang*, Preliminary Quantitative Profile of Differential Expression between Rat L6 Myoblasts and Myotubes by Stable Isotope Labeling by Amino acids in Cell Culture, Proteomics, 2009, 9(5), 1274-1292.

4. Shi L, Zhang J, Wu P, Feng K, Li J, Xie Z, Xue P, Cai T, Cui Z, Chen X, Hou J, Zhang J, Yang F*., Discovery and identification of potential biomarkers of pediatric Acute Lymphoblastic Leukemia. Proteome Sci. 2009,7(7):1-12.

5. Xie X, Chen Y, Xue P, Fan Y, Deng Y, Peng G, Yang F*, Xu T*, RUVBL2, a novel AS160-binding protein, regulates insulin-stimulated GLUT4 translocation. Cell Res. 2009, 1-8.

6. Zhang X, Chen Q, Feng J, Hou J, Yang F, Liu J, Jiang Q, Zhang C. Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the {gamma}TuRC to the centrosome., J Cell Sci. 2009, 122(13): 2240-2251.

7. Li Y, Li C, Xue P, Zhong B, Mao AP, Ran Y, Chen H, Wang YY, Yang F, Shu HB., ISG56 is a negative-feedback regulator of virus-triggered signaling and cellular antiviral response., Proc Natl Acad Sci, 2009, 106(19): 7945-7950.

8. Fan Y, Shi L, Yu J, Yang FQ*, Wang JX*, Discovery and identification of potential biomarkers of papillary thyroid carcinoma, Molecular Cancer, 2009, 28; 8:79.

9. W. He, Y. Zhao, C. Zhang, L. L. An, Z. S. Hu, Y. Liu, L. Han, L. Bi, Z. S. Xie, P. Xue, F. Q. Yang* and H. Y. Hang*, Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1, Nucleic Acids Research, 2008, 1-12.

10. X. Duan, Q. Lu, P. Xue, Z. Dong, F. Q. Yang, N. L. Wang, Proteomic analysis of aqueous humor from patients with myopia, Molecular Vision, 2008, 14, 370-377.

11. D. A. Davis, K. E. Singer, M. Sierra, M. Narazaki, F. Q. Yang, H. M. Fales, R. Yarchoan and G. Tosato, Identification of Carboxypeptidase N as an Enzyme Responsible for C-Terminal Cleavage of Stromal Cell-Derived Factor-1a in the Circulation”, Blood ,105(12)，4561-4568 (2005).

12. M. Sierra1, F. Q. Yang1 (co-first author), M. Narazaki, O. Salvucci, D. Davis, R. Yarchoan, J. Farber, H. Zhang, H. M. Fales, and G. Tosato, Differential processing of stromal-derived factor-1 Alpha and Beta explains functional diversity", Blood, 103 (7) 2452-2459 (2004).

13. F. Q. Yang and Y. Ito, On-line flow-injection dectection system and its application to the measurement of ethanol transfer and osmosiss rate in centrifugal precipitation chromatography.  J. Liq. Chroma. Rel. Tech., 27, 2979-2985 (2004).

14. Y. Ito, F. Q. Yang, P. E. Fitze, and J. V. Sullivan, Improved column design for high-speed countercurrent chromatography. J. Chromatogr. A. 1017 (2003) 71-81.

15. F. Q. Yang and Y. Ito, “Separation of alkaloids by countercurrent chromatography”,  The Encyclopedia of Chromatogrphy (online) Marcel Dekker, 2003, pp1-5.

16. Y. Ito, F. Q. Yang, P. E. Fitze, and J. V. Sullivan, Spiral disk assembly for HSCCC: Column design and basic studies on chromatographic resolution and stationary phase retention.  J. Liq. Chroma. Rel. Tech., 26 (2003) 1355-1372.

17. F. Q. Yang, Y. Ito, A novel method for the fractionation of dextran by centrifugal precipitation chromatography”, Anal. Chem., 74 (2002) 440-445.

18. F. Q. Yang and Y. Ito, “Preparative separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from crude alkaloids of sample Aconitum sinomontanum Nakai by high-speed countercurrent chromatography, J. Chromatogr. A. 943 (2002) 219-225.

19. F. Q. Yang, Y. Ma, Y. Ito, Separation and purification of isoflavones from a crude extract from soybean by high-speed countercurrent chromatography, J. Chromatogr. A. 928 (2001)163-170.

20. F. Q. Yang, Y. Ito, "pH-Zone-refining countercurrent chromatography of Lappaconitine from Aconitum sinomontanum Nakai: I. Separation of prepurified extract", J. Chromatogr. A. 923 (2001) 281-285.

21. F. Q. Yang, T. Y. Zhang, G. Q. Xu, F. E. Chou, Y. Ito, pH-modulated stepwise elution countercurrent chromatography CCC and its application on the preparative separation of hydroxyanthrquinones compounds from traditional Chinese medicine herbs. J. Liq. Chrom. & Relat. Tech. Special Issue on CCC,  24 (2001) 1617-1628.

22. F. Q. Yang, T. Y. Zhang, Y. Ito, Large-scale separation of resveratrol, anthraglycoside A and anthraglycoside B from Polygonum cuspidatum Sieb. Et Zucc. by high-speed countercurrent chromatography. J. Chromatogr. A,  919 (2001) 443-448.

23. F. Q. Yang, T. Y. Zhang, Q. H. Liu, G. Q. Xu, Y. B. Zhang, Y. Ito, Preparative isolation and purification of notopterol and isoimperatorin from Chinese traditional medicine Notopterygium forbessi Boiss (Qianghuo in Chinese) by high-speed countercurrent chromatography.  J. Chromatogr. A, 883 (2000) 67-73.

24. F. Q. Yang, T. Y. Zhang, Y. Ito, Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by HSCCC using pH-modulated stepwise elution.J. Chromatogr. A, 858 (1999) 103-107.

25. F. Q. Yang, J. Quan, T. Y. Zhang, Y. Ito, Application of analytical and preparative high-speed countercurrent chromatography in the separation of alkaloids from Coptis chinensis Franch, J. Chromatogr. A, 829 (1998) 137-141.

26. F. Q. Yang, J. Quan, T. Y. Zhang, Y. Ito, "Preparative separation of alkaloids from the root of Sophora flavescens Ait by pH-zone-refining countercurrent chromatography". J. Chromatogr., A., 822 (1998) 316-320.

27. F. Q. Yang, T. Y. Zhang, and Y. Ito, Multidimensional countercurrent chromatography and is applications.  J. Chromatogr. A., 803, 298-301 (1998).

28. F. Q. Yang, T. Y. Zhang, B. X. Mo, and Y. Ito, Preparative separation and purification of kaempferol, isorhamnetin and quercetin by high-speed countercurrent chromatography.  J. Liq. Chrom. & Rel. Technol., 21(1&2), 209-216, 1998.

29. J. S. Edmonds, Y. Shibata, F. Q. Yang and M. Morita, Isolation and synthesis of 1-Deoxy-1-dimethylarsinoylribitol-5-sulfate, a natural constituent of Chondria crassicaulis and other red algae, Tetrahedron Letters, 38(33), 5819, 1997.

30. F. Q. Yang, Q. Y. Ou, W. L. Yu, Semi-preparative separation of araxerylacetate and coumarins from Artemisia dalailamae Kraschen by high-speed countercurrent chromatography. J. Liq. Chromatogr., 18(2), 395-403, 1995.